Plasmid_Backbone
pMH

Part:BBa_K1830000:Design

Designed by: Mathieu Husser   Group: iGEM15_Concordia   (2015-08-06)

pET15BB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3530
    Illegal XbaI site found at 47
    Illegal SpeI site found at 213
    Illegal PstI site found at 288
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3530
    Illegal SpeI site found at 213
    Illegal PstI site found at 288
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3530
    Illegal BglII site found at 3818
    Illegal BamHI site found at 162
    Illegal XhoI site found at 157
    Illegal XhoI site found at 1305
    Illegal XhoI site found at 2197
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3530
    Illegal XbaI site found at 47
    Illegal SpeI site found at 213
    Illegal PstI site found at 288
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3530
    Illegal XbaI site found at 47
    Illegal SpeI site found at 213
    Illegal PstI site found at 288
    Illegal NgoMIV site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

iGEM pSB1C3

LacI operon taken from pET15b for inducible expression

MCS designed by iGEM Concordia 2015


Source

iGEM Concordia 2015, M. Husser, L. Nacross, A. Sundarakhrishnan

iGEM biobrick standards

References